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Buffalo County, WI is located between Onalaska and Eau Claire in the western part of the state toward the center with Mississippi River & Chippewa River forming some of its borders. Buffalo is more of an area set in hills, valleys, rivers, streams with Five-Mile Bluff Prairie State Natural Area, Nelson-Trevino Bottoms State Natural Area, Buena Vista Park Merrick State Park and a couple communities including; Mondovi, Modena and Gilmanton. It's surrounded by 3 other Counties; Pepin, Eau Claire and Trempealeau. Buffalo County, Wisconsin GIS MAP Information!